CONSOLIDATE DNA FINGERPRINTING
REPORT
Project title: DNA Fingerprinting 38 crops with
5 to 8 RAPD markers each.
Introduction:
In collaboration with Mr. Mahendra Kumar Trivedi, Bangalore Genei carried out a DNA mapping project. The
aim of this project was to observe the impact of Mr.
Mahendra Kumar Trivedi's treatment modality on various
groups of crop lines and to analyze the percentage of
DNA Polymorphism (Mutation) observed between Treated
and Untreated samples of 38 crops.
The selection of the crop lines & the treatment
part of the said project was designed and carried out
by Mr. Mahendra Kumar Trivedi (Mumbai) himself. Our
laboratory was used to detect the polymorphism (mutation)
on consultancy basis.
Dr Avijit Roy (Manager - R&D) & Mr. Mehul Rajgor
(Designation - Assistant Executive Manager - Sales)
conducted this project.
Objective:
The objective of this project was to genotype the 2
samples (Treated vs. Untreated) of the same line with
5 to 8 RAPD markers as per the requirement of individual
crop.
Duration of the project: 3 months
Setting:
Bangalore Genie, Division of Sanmar Speciality Chemicals
Ltd,
No.6, 6 Main, BDA Industrial Suburb,
Peenya, Bangalore- 560 058, Karnataka - India
Samples:
Both Control (untreated) and Treated samples were procured
from Mr. Mahendra Kumar Trivedi in two separate groups.
Group A - Untreated (Control) Samples (Series
1) - 38 samples
Group B - Treated Samples (Series 2) - 38 samples
All varieties of Control and Treated seeds were sowed
in the soil separately. After germination when plants
reached the appropriate stage, leaf discs were harvested
from each plant. DNA extractions were performed according
to standard CTAB method (Sambrook et al; 2000). Bangalore
Genei selected Markers of each crop as per the requirement
of individual crop and PCR reactions were amplified
following Bangalore Genei standard protocol for RAPD
on all samples.
The percentage of the true Polymorphism (Mutation)
observed in Control and Treated samples have been recorded
in a tabular form as follows:
Click the crop name for report
Conclusion:
The treatment modality of Mr. Mahendra Kumar Trivedi
is not known to us, the company is only concerned about
detecting the DNA Polymorphism (Mutation) in Control
& Treated lines of 38 crops.
Approved by: Dr. Avijit Roy
Manager R & D
Bangalore Genei.
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