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Soybean Report

Dr. Madhuri Sharon
Managing Director

Monad Nanotech Pvt. Limited
A702 Bhawani Towers,
Adishankaracharya Marg,
Powai Mumbai 400 076
Fax No +91 (0251) 2231029,
Tel No +91 (022) 25790633
Mobile No 9821238584
Email: monadnt@yahoo.com
Web: www. monandnano.com
Comp.Reg. No. U 24230 MH 2004 PTC 144302
 

SOYBEAN Experimental Data
 
GROWTH GERMINATION OF SEEDLINGS
 
  • Blessed as well as Control Rice seeds (Oryza sativa cv, 644) were soaked for 6hrs in Distilled water.
  • Seeds were germinated on moist tissue papers in dark.
  • % seeds showing radical emergence was recorded
  • % seeds germination was recorded
  • Length of root and shoot was also recorded.
CONCISED DATA OF SEEDLING GROWTH
 
Treatment
Germinated By
Germination %
Length
(cm ± S.E.) of
IAA µg/g in
Shoot
Root
Shoot
Root
Control
7th day
94.7%
4.654
9.239
3.0

1.43
Treated
7th day
98.0%
5.26
10.7

4.36

2.5
 
GLUTATHIONE in Soybean LEAVES
 
Glutathione from
Concentration of endogenous level of Glutathione
Remarks
Sample1
Sample 2
Sample 3
Sample 4
Mean
CONTROL
1.2
1.05
1.34
1.21
1.2

28.33% INCREASE
Blessed
1.58
1.61
1.37
1.6
1.54
 
IAA STAANDARD CURVE DATA
 
Standard IAA
µg / ml
1
2
3
Mean
1
0.004
0.005
0.005
0.005
2
0.008
0.014
0.011
0.011
3
0.015
0.015
0.015
0.015
4
0.03
0.029
0.031
0.03
5
0.047
0.052
0.043
0.473
6
0.067
0.083
0.065
0.716
7
0.095
0.1
0.099
0.98
8
0.13
0.123
0.125
0.126
9
0.168
0.168
0.171
0.169
10
0.192
0.2
0.197
0.196
 
IAA CONTENT IN SHOOT AND ROOTS OF RICE SEEDLINGS
 
IAA in Soybean ROOT
 
TREATMENT
IAA µg/g
MEAN IAA µg/g
% INCREASE in Blessed
Sample1
Sample 2
Sample 3
CONTROL
1.6
1.6
1.1
1.43
74.82%
Blessed
2.1
2.9
2.5
2.5
 
IAA in Soybean SHOOT
 
TREATMENT
IAA µg/g
MEAN IAA µg/g
% INCREASE in Blessed
Sample1
Sample 2
Sample 3
CONTROL
2.9
2.9
3.3
3.03
43.89 %
Blessed
4.5
5.1
3.5
4.36
 
PROCEDURES FOLLOWED FOR
 
IAA EXTRACTION & ANALYSIS
 
  • IAA was analyzed using Tang and Bonner's method
  • Freshly prepared Salkowski's reagent was used for detection
  • 200 mg plant tissue was grinded with 5 ml of 80% chilled methanol
  • Extract was filtered through Whatmann paper no. 1
  • Final volume of extract was made up to 10 ml using 80% chilled methanol
  • Optical density was measure after 30 minutes at 530 nm.
 
GLUTATHIONE EXTRACTION
 
The glutathione extraction procedure was as follows:
  • 5 gm of leaves was crushed in 5 ml of 80% chilled methanol (as a solvent)
  • Extract was sonicated for 10 min.
  • After sonication the total volume was adjusted to 5 ml.
  • Then 1ml of 5% tricholoroacetic acid was added to the extract
  • This sample was used for glutathione analysis.
GLUTATHIONE ANALYSIS
 
  • The glutathione levels were estimated by Moron et.al's (1979) method.
  • Trichloroacetic acid was taken as blank.
  • Procedure given in Biochimica et Biophysica acta, 582, 1979, 67-78 was followed.
INFERENCE
 
Very significant increase in germination as well as seedling growth associated with increased level of auxin (IAA) in both root and shoot of blessed plants suggest an early establishment of healthy plant
 
Absence of any type of disease (i.e. viral, fungal and insect) was noticed in blessed plants, which is supported by an increased level of glutathione in them.
 
    
Dr. Madhuri Sharon         
 
GROWTH GERMINATION OF CONTROL SEEDLINGS
 
Sr. No.
Shoot Length Control (cm)
Root Length Control (cm)
1
3.1
8.5
2
5.6
6.1
3
7.6
10.5
4
4.0
10.9
5
6.2
11.5
6
3.1
6.1
7
2.3
8.9
8
4.0
7.8
9
2.5
7.6
10
4.6
7.6
11
3.8
9.0
12
3.4
9.6
13
5.0
8.8
14
7.4
8.9
15
5.3
5.6
16
5.9
9.0
17
4.7
7.2
18
5.2
11.5
19
6.7
11.2
20
6.3
5.4
21
7.3
12.6
22
6.9
15.8
23
5.4
10.8
24
5.9
9.6
25
5.6
9.6
26
8.3
9.0
27
10.0
16.8
28
10.1
15.2
29
5.8
13.3
30
8.0
16.7
31
7.1
6.2
32
6.8
9.5
33
8.1
13.7
34
5.7
11.2
35
6.6
7.0
36
5.8
9.9
37
5.1
8.5
38
5.7
7.7
39
5.8
9.6
40
7.8
14.8
41
5.5
9.6
42
6.4
11.3
43
4.1
8.3
44
5.1
10.7
45
3.1
9.5
46
3.3
8.8
47
3.4
10.0
48
4.3
13.5
49
3.7
8.7
50
5.2
5.0
51
6.9
9.3
52
5.1
8.6
53
5.1
9.9
54
4.4
11.6
55
4.2
12.9
56
6.1
9.8
57
3.0
4.0
58
2.9
8.1
59
2.1
8.8
60
4.2
6.7
61
3.1
10.1
62
6.0
10.7
63
3.4
8.0
64
3.2
5.3
65
2.3
9.3
66
6.7
13.2
67
6.8
16.7
68
2.8
7.1
69
3.5
7.3
70
3.5
6.0
71
2.6
6.3
72
4.5
8.6
73
1.6
5.6
74
6.4
5.5
75
3.6
8.2
76
3.4
3.3
77
4.6
10.4
78
3.4
10.7
79
4.1
7.3
80
3.0
10.1
81
5.1
10.6
82
3.1
10.1
83
3.8
9.5
84
5.0
10.3
85
3.6
7.6
86
2.7
6.7
87
3.5
8.0
88
3.2
9.4
89
1.0
6.3
90
3.7
6.5
91
2.1
9.5
92
2.8
10.1
93
4.5
7.3
94
2.0
7.9
95
1.7
7.1
96
2.7
6.9
97
3.0
10.7
98
3.6
9.3
99
4.3
8.7
100
2.9
7.4
Total
465.4
923.9
Average
4.654
9.239
Total seeds = 151
did not germinated = 8
only radicals = 14
Good germination = 129
Percentage germination 94.7%
 
GROWTH GERMINATION OF BLESSED SEEDLINGS
 
Sr. No.
Shoot Length Blessed (cm)
Root Length Blessed (cm)
1
12.2
15.4
2
10.0
15.1
3
3.5
8.3
4
4.5
8.9
5
10.7
10.6
6
6.5
10.8
7
4.3
8.6
8
1.2
4.4
9
8.5
13.9
10
5.4
11.6
11
8.0
11.5
12
8.5
11.1
13
7.0
11.8
14
3.7
10.0
15
9.3
12.0
16
3.8
6.2
17
3.6
10.7
18
10.0
9.6
19
8.2
14.0
20
7.4
13.5
21
4.8
14.1
22
7.1
10.9
23
6.6
8.0
24
5.7
12.2
25
6.7
4.2
26
1.4
10.0
27
8.1
13.5
28
6.6
11.9
29
3.3
7.9
30
3.1
7.0
31
2.0
11.3
32
4.7
12.6
33
9.1
14.4
34
2.7
3.6
35
1.9
7.6
36
5.1
7.7
37
5.1
12.6
38
2.3
9.8
39
5.6
10.8
40
2.8
11.8
41
4.0
8.8
42
3.0
10.1
43
10.1
9.5
44
4.5
13.0
45
10.4
13.2
46
10.0
16.4
47
5.4
14.8
48
4.3
11.0
49
3.6
12.5
50
10.6
12.4
51
8.3
15.8
52
6.4
15.6
53
3.7
12.5
54
10.7
15.0
55
6.2
12.7
56
5.3
12.4
57
4.6
11.3
58
5.3
14.4
59
3.5
10.6
60
3.6
10.9
61
5.6
14.2
62
4.2
6.0
63
3.9
9.3
64
2.9
10.3
65
2.7
8.6
66
2.5
8.3
67
3.5
11.5
68
6.1
11.2
69
4.1
6.5
70
3.3
8.5
71
4.0
10.0
72
3.7
7.1
73
6.8
14.8
74
5.1
9.2
75
4.5
4.4
76
4.5
12.1
77
11.7
15.7
78
4.7
13.1
79
7.2
13.0
80
5.8
12.8
81
7.0
12.5
82
3.5
10.0
83
8.3
13.5
84
3.5
8.1
85
2.6
9.2
86
6.0
12.1
87
3.2
9.8
88
3.4
10.1
89
3.1
8.2
90
2.5
6.7
91
2.4
7.3
92
3.4
9.0
93
2.5
8.8
94
3.5
7.3
95
3.2
8.5
96
3.0
10.2
97
3.1
8.4
98
4.5
12.3
99
3.4
12.3
100
2.9
6.6
Total
526.3
1070.3
Average
5.26
10.7
Total seeds = 150
did not germinated = 3
only radicals = 00
Good germination = 147
Percentage
98%
germination
 
  

   
 
     
     
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