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Rice Report

Dr. Madhuri Sharon
Managing Director

Monad Nanotech Pvt. Limited
A702 Bhawani Towers,
Adishankaracharya Marg,
Powai Mumbai 400 076
Fax No +91 (0251) 2231029,
Tel No +91 (022) 25790633
Mobile No 9821238584
Email: monadnt@yahoo.com
Web: www. monandnano.com
Comp.Reg. No. U 24230 MH 2004 PTC 144302
 

RICE Experimental Data
 
GROWTH GERMINATION OF SEEDLINGS
 
  • Blessed as well as Control Rice seeds (Oryza sativa cv, 644) were soaked for 6hrs in Distilled water.
  • Seeds were germinated on moist tissue papers in dark.
  • % seeds showing radical emergence was recorded (Table 1)
  • % seeds germination was recorded (Table 1)
  • Length of root and shoot was also recorded.
CONCISED DATA OF SEEDLING GROWTH
 
Treatment
Germinated By
Germination %
Length
(cm ± S.E.) of
IAA µg/g in
Glutathione in Leaves
Shoot
Root
Shoot
Root
Control

5th day
100%
5.7
± 0.013
8.8
± 0.05
4.03
0.87
0.163
Treated
3rd day
100%
5.8
± 0.021
8.9
±0.05
4.85
1.80
1.205
 
GLUTATHIONE IN RICE LEAVES
 
Glutathione extract from
Concentration of endogenous level of Glutathione (mM)
Remarks or Inference
Control (Leaves from Seedlings)
0.163
639.26 % Increase
Blessed (Leaves from Seedlings)
1.205
Control (Leaves from Mature plants grown in field)
0.521
56.23% Increase
Blessed (Leaves from Mature plants grown in field)
0.814
 
IAA STAANDARD CURVE DATA
 
Standard IAA
µg / ml
1
2
3
Mean
1
0.004
0.005
0.005
0.005
2
0.008
0.014
0.011
0.011
3
0.015
0.015
0.015
0.015
4
0.03
0.029
0.031
0.03
5
0.047
0.052
0.043
0.473
6
0.067
0.083
0.065
0.716
7
0.095
0.1
0.099
0.98
8
0.13
0.123
0.125
0.126
9
0.168
0.168
0.171
0.169
10
0.192
0.2
0.197
0.196
 
IAA CONTENT IN SHOOT AND ROOTS OF RICE SEEDLINGS
 
IAA in RICE ROOT
 
TREATMENT
IAA µg/g
MEAN IAA µg/g
% INCREASE in Blessed
Sample1
Sample 2
Sample 3
CONTROL
0.7
0.8
1.1
0.87
106.89%
Blessed
1.4
1.8
2.2
1.8
 
IAA in RICE SHOOT
 
TREATMENT
IAA µg/g
MEAN IAA µg/g
% INCREASE in Blessed
Sample1
Sample 2
Sample 3
CONTROL
4.0
4.3
3.8
4.03
20.34 %
Blessed
5.1
5.3.
4.15
4.85
 
PROCEDURES FOLLOWED FOR
 
IAA EXTRACTION & ANALYSIS
 
  • IAA was analyzed using Tang and Bonner's method
  • Freshly prepared Salkowski's reagent was used for detection
  • 200 mg plant tissue was grinded with 5 ml of 80% chilled methanol
  • Extract was filtered through Whatmann paper no. 1
  • Final volume of extract was made up to 10 ml using 80% chilled methanol
  • Optical density was measure after 30 minutes at 530 nm.
 
GLUTATHIONE EXTRACTION
 
The glutathione extraction procedure was as follows:
  • 5 gm of leaves was crushed in 5 ml of 80% chilled methanol (as a solvent)
  • Extract was sonicated for 10 min.
  • After sonication the total volume was adjusted to 5 ml.
  • Then 1ml of 5% tricholoroacetic acid was added to the extract
  • This sample was used for glutathione analysis.
GLUTATHIONE ANALYSIS
 
  • The glutathione levels were estimated by Moron et.al's (1979) method.
  • Trichloroacetic acid was taken as blank.
  • Procedure given in Biochimica et Biophysica acta, 582, 1979, 67-78 was followed.
INFERENCE
 
Very significant increase in seedling growth associated with increased level of auxin (IAA) in both root and shoot seedlings of blessed plants suggest an early establishment of healthy plant.
 
In rice a Bacterial infection by Xanthomonas campestris is very common.
Though it was noticed in all the control plants, it was totally absent in blessed plants; hence in both control and blessed plants glutathione analysis was done.
 
Blessed plants showed 639.26% increase in young seedling which remained increased till maturity and was recorded to be 56.23% more than control. Showing increased immunity.
 
    
Dr. Madhuri Sharon         
 
GROWTH GERMINATION OF SEEDLINGS
 
Sr. No.
Length (cm) of 10 days old plant
Number of roots
Root
Shoot
Blessed (B)
Control ©
Blessed (B)
Control ©
Blessed (B)
Control ©
1
14
10.7
5.1
4.6
7
6
2
11.3
14.7
6.5
6.7
8
6
3
8.7
11.6
7.5
7.4
7
6
4
10.3
7.6
5.6
6.8
8
7
5
13.5
10.1
7.3
7.5
10
7
6
10.3
8.8
4
5.5
9
7
7
11.1
8.9
7.3
5.6
8
8
8
7.3
10.5
5.1
6.5
7
7
9
10.4
12.5
5.5
6
8
5
10
11.5
8.3
6.7
4.5
7
7
11
5.5
9.4
4.2
6.2
6
5
12
9.3
7.1
6
5.1
7
7
13
8.6
9.5
7.1
6.6
7
7
14
9.1
10.1
7.3
7.4
9
6
15
8
13.2
6.2
4.5
7
5
16
8.5
7.1
7.3
5.9
9
6
17
12.4
11.5
4.6
5.4
8
7
18
10.4
10.1
6.4
6.8
8
6
19
11.1
9.4
5.2
6.5
9
4
20
10.7
9.5
6.9
4.1
7
8
21
9.2
9.4
5.9
5.4
6
7
22
5.2
10.6
5.5
5.5
7
8
23
6.8
7.8
7.1
5.3
8
6
24
10.1
9.6
5.4
5.3
7
7
25
11.1
7.4
6.5
5.6
8
5
26
9.2
12.1
6.6
4.9
7
6
27
8.4
10.1
6.1
6.4
6
5
28
7.3
7.4
6.7
5.8
5
6
29
8.4
3.6
6.6
5.1
5
6
30
7.2
11.8
5.7
6.4
8
7
31
9.6
12.8
5.7
5.8
7
7
32
11.7
11.4
5.8
6.3
6
6
33
8.8
8.5
7
6.4
7
5
34
9.1
8.6
7.2
5.8
6
4
35
7.2
7.1
4.9
3.8
7
6
36
7.7
9.3
6.2
5.3
7
8
37
6.7
8.7
4
4.9
9
7
38
7.5
9.6
6
6.3
7
8
39
9.8
8.5
7.7
6.6
7
4
40
10.6
13.1
5.2
5.9
7
6
41
10.7
8.1
4.8
6.3
8
7
42
10.2
4.5
6
4.6
8
5
43
12.1
5.1
5.1
5.6
9
4
44
9.1
9.6
6.7
4.6
7
6
45
12.3
9.5
6.2
6.8
8
8
46
6.1
13.2
5.3
6.9
6
6
47
9.7
10.2
6.1
6.2
8
7
48
8.6
8.3
3.5
6.2
7
4
49
9.3
8.2
6.7
5.3
7
7
50
10.6
9.5
5.4
4.4
6
7
51
12.3
8.5
4.5
4.7
6
5
52
7.6
9.1
6.7
5.4
6
6
53
5.1
10
2.6
5.2
6
6
54
10.5
11.3
5.5
6.1
8
8
55
7.9
8.4
5.5
5.7
5
5
56
12.2
6.5
5.3
4.5
6
6
57
7.1
7.5
4.6
5.1
6
7
58
4.1
9.6
4.6
5.7
8
8
59
10.5
9.8
4.7
6.1
6
8
60
11.1
7.3
5.2
6.1
7
7
61
8.4
7.7
6.1
6.7
10
6
62
7.4
6.4
5.7
6.8
7
5
63
11.8
10.2
5.3
5.1
6
6
64
7.3
5.9
4.9
4.3
6
6
65
5.8
6.3
6.2
5.4
7
6
66
5.5
5.8
4.7
4.7
7
5
67
11.3
8.7
6
6.1
6
6
68
7.9
6.9
5.1
4.7
7
5
69
8.4
7.1
5.8
4.4
7
7
70
12.3
8.7
5.4
5.1
6
7
71
10.8
5.7
5.3
4.8
5
7
72
11.3
10.3
5.2
5.9
4
6
73
10.2
11.1
5.4
5.3
8
4
74
6.7
6.4
4.7
4.9
7
8
75
6.4
11.3
5.5
6.1
7
6
76
7.6
9.4
4.4
5.5
5
6
77
9.4
8.6
5.6
6.1
7
8
78
6.7
7.9
5.1
5.4
9
6
79
7.1
6.8
4.8
4.7
7
5
80
12.2
9.6
5.6
5.3
6
5
81
6.5
9.7
4.6
5.4
6
7
82
7.4
6.7
5.4
5.9
7
7
83
4.7
8.1
3.8
4.9
7
6
84
4.4
7.9
5.6
6.1
8
5
85
6.7
6.7
4.9
5.7
4
7
86
6.5
9.3
6.4
5.4
7
7
87
8.9
9.7
5.7
6.3
7
7
88
8
8.4
4.5
5.7
5
8
89
14.6
8.7
6.1
6.4
5
6
90
13.5
5.1
6.2
4.7
8
4
91
5.8
5.3
2.7
6.6
6
8
92
7.7
6.8
5.1
6.4
6
6
93
4.3
8.9
3.3
5.6
7
5
94
7.5
9.9
3.9
5.7
4
8
95
9.4
9.3
5.3
6.7
7
7
96
5.3
8.6
5.4
4.9
4
5
97
8.9
8.7
5.1
5.4
4
6
98
8.6
9.5
6.2
5.6
8
7
99
8.7
10.1
6.2
6.5
8
6
100
12.1
8.7
5.3
5.5
8
6
MEAN
8.9
8.8
5.5
5.6
6.9
6.2
  
 
 

   
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