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Mustard Report

Dr. Madhuri Sharon
Managing Director

Monad Nanotech Pvt. Limited
A702 Bhawani Towers,
Adishankaracharya Marg,
Powai Mumbai 400 076
Fax No +91 (0251) 2231029,
Tel No +91 (022) 25790633
Mobile No 9821238584
Email: monadnt@yahoo.com
Web: www.monandnano.com
Comp.Reg. No. U 24230 MH 2004 PTC 144302
 

Mustard Experimental Data
 
CONCISED DATA OF SEEDLING GROWTH AND GERMINATION
 
Treatment
Germinated By
Germination %
Length
(cm ± S.E.) of
IAA µg/g in
Glutathione in Leaves
Shoot
Root
Shoot
Root
Control
7 - 10th day
65%
8.891
± 0.014
5.660
± 0.06
17.7
25.3
0.2917 mM
Treated
5th day
100%
9.845
± 0.020
5.751
±0.047
20.0
29.3
0.8947 mM
 
IAA STAANDARD CURVE DATA
 
Standard IAA
µg / ml
1
2
3
Mean
1
0.004
0.005
0.005
0.005
2
0.008
0.014
0.011
0.011
3
0.015
0.015
0.015
0.015
4
0.03
0.029
0.031
0.03
5
0.047
0.052
0.043
0.473
6
0.067
0.083
0.065
0.716
7
0.095
0.1
0.099
0.98
8
0.13
0.123
0.125
0.126
9
0.168
0.168
0.171
0.169
10
0.192
0.2
0.197
0.196
 
IAA CONTENT IN SHOOT AND ROOTS OF SEEDLINGS
 
MUSTARD
wt (mg)
mean O.D.
iaa/10 gm
Remarks
root blessed
220
0.284
200
13% increase
root control
180
0.248
177
shoot blessed
210
0.718
295
16.60% increase
shoot control
280
0.102
253
 
GLUTATHIONE IN LEAVES OF MUSTARD
 
Mustard
a
b
c
d
MEAN
Remark
CONTROL
0.2917
0.2891
0.2798
0.3062
0.2917 mM
206.71% Increase
TREATED
0.9154
0.8875
0.8764
0.8995
0.8947 mm
 
PROCEDURES FOLLOWED FOR
 
IAA EXTRACTION & ANALYSIS
 
  • IAA was analyzed using Tang and Bonner's method
  • Freshly prepared Salkowski's reagent was used for detection
  • 200 mg plant tissue was grinded with 5 ml of 80% chilled methanol
  • Extract was filtered through Whatmann paper no. 1
  • Final volume of extract was made up to 10 ml using 80% chilled methanol
  • Optical density was measure after 30 minutes at 530 nm.
 
GLUTATHIONE EXTRACTION
 
The glutathione extraction procedure was as follows:
  • 5 gm of leaves was crushed in 5 ml of 80% chilled methanol (as a solvent)
  • Extract was sonicated for 10 min.
  • After sonication the total volume was adjusted to 5 ml.
  • Then 1ml of 5% tricholoroacetic acid was added to the extract
  • This sample was used for glutathione analysis.
GLUTATHIONE ANALYSIS
 
  • The glutathione levels were estimated by Moron et.al's (1979) method.
  • Trichloroacetic acid was taken as blank.
  • Procedure given in Biochimica et Biophysica acta, 582, 1979, 67-78 was followed.
INFERENCE
Very significant increase in germination, seedling growth associated with increased level of auxin (IAA) in both root and shoot of seedlings of blessed plants is 13% &16.60% respectively which suggests an early establishment of healthy plant.
 
Absence of any type of disease (i.e. viral, fungal and insect) was noticed in blessed plants, which is supported by 206.71% increased level of glutathione in them.
 
    
Dr. Madhuri Sharon         
 
I. GROWTH & GERMINATION OF SEEDLINGS
 
Sr. No.
Length (cm) of 10 days old plant
Number of roots
Root
Shoot
Blessed (B)
Control (C)
Blessed (B)
Control (C)
Blessed (B)
Control (C)
1
15
11.7
6.1
5.6
8
7
2
10.3
13.7
6.5
6.7
8
6
3
9.7
10.6
6.5
6.4
7
6
4
9.3
8.6
5.6
5.8
8
7
5
14.5
9.1
7.3
6.5
9
7
6
9.3
9.8
5.0
5.5
9
7
7
12.1
9.9
6.3
5.6
8
7
8
6.3
9.5
5.1
5.5
7
7
9
11.4
11.5
5.5
6.0
8
6
10
10.5
9.3
6.7
5.5
7
8
11
6.5
8.4
5.2
6.2
6
6
12
8.3
8.1
6.0
5.1
7
7
13
9.6
9.5
6.1
5.6
7
7
14
8.1
10.1
7.3
6.4
9
6
15
9.0
12.2
6.2
5.5
7
6
16
8.5
8.1
6.3
5.9
9
6
17
13.4
10.5
5.6
5.4
8
8
18
9.4
9.1
6.4
5.8
8
6
19
12.1
10.4
5.2
5.5
9
5
20
9.7
8.5
6.9
5.1
7
7
21
10.2
8.4
5.9
5.4
6
7
22
4.2
9.6
5.1
5.5
7
7
23
7.8
8.8
7.5
5.3
8
6
24
9.1
8.6
5.5
5.3
7
7
25
12.1
8.4
6.4
5.6
8
6
26
8.2
11.1
6.6
5.9
7
6
27
9.4
9.1
6.1
6.4
6
6
28
6.3
8.4
6.5
5.8
5
6
29
9.4
4.6
6.8
5.1
5
6
30
6.2
10.8
5.5
6.4
8
7
31
10.6
12.8
5.9
5.8
7
7
32
10.7
11.4
5.6
6.3
7
6
33
9.8
8.5
7.2
6.4
7
6
34
8.1
8.6
7.0
5.8
7
6
35
8.2
8.1
4.9
4.8
7
6
36
6.7
8.3
6.4
5.3
7
7
37
7.7
8.7
4.0
5.9
8
7
38
6.5
9.6
6.0
6.3
7
7
39
10.8
9.5
6.7
5.6
7
4
40
9.6
12.1
6.2
5.9
7
6
41
11.7
7.1
5.8
6.3
8
7
42
9.2
5.5
5.0
5.6
8
5
43
12.1
6.1
5.1
5.6
8
4
44
8.1
8.6
6.7
5.6
7
6
45
11.3
10.5
6.2
5.8
8
8
46
5.1
12.2
5.3
5.9
6
6
47
10.7
9.2
6.1
6.2
7
7
48
7.6
9.3
4.5
6.2
7
4
49
10.3
9.2
5.7
5.3
7
7
50
8.6
8.5
5.4
5.4
6
7
51
13.3
8.5
4.5
5.7
6
5
52
6.6
9.1
5.7
5.4
6
6
53
6.1
10.0
3.6
5.2
6
6
54
9.5
11.3
5.5
6.1
7
8
55
8.9
9.4
5.5
5.7
6
5
56
11.2
5.5
5.6
5.5
6
6
57
8.1
8.5
4.3
5.1
7
7
58
3.1
8.6
4.6
5.7
8
8
59
11.5
8.8
4.7
6.1
6
8
60
10.1
8.3
5.2
6.1
7
7
61
9.4
8.7
6.1
5.7
9
6
62
6.4
7.4
5.7
5.8
7
5
63
10.8
9.2
5.3
5.1
6
6
64
6.3
6.9
4.9
5.3
7
6
65
6.8
6.3
5.2
5.4
7
6
66
4.5
5.8
5.7
67
12.3
7.7
6.0
68
6.9
7.9
5.1
69
9.4
7.1
5.5
70
11.3
8.7
5.7
71
12.8
6.7
5.3
72
10.3
9.3
5.0
73
11.2
10.1
5.6
74
5.7
7.4
4.7
75
7.4
10.3
5.5
76
6.6
10.4
5.0
77
10.4
8.6
5.0
78
5.7
7.9
5.1
79
8.1
7.8
4.8
80
11.2
8.6
5.6
81
7.5
8.7
4.6
82
6.4
7.7
5.4
83
5.7
8.1
4.8
84
4.4
7.9
4.6
85
7.7
7.7
5.9
86
5.5
9.3
5.4
87
9.9
8.7
5.7
88
7.0
9.4
4.8
89
13.6
7.7
6.0
90
12.5
6.1
6.0
91
6.8
5.3
4.7
92
6.7
6.8
5.1
93
5.3
8.9
4.3
94
6.5
9.9
4.9
95
10.4
9.3
4.3
96
4.3
8.6
4.4
97
9.9
8.7
4.1
98
7.6
9.5
5.2
99
9.7
9.1
6.2
100
11.1
9.7
5.3
 
  

   
 
 
     
     
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