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Cotton Report
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Dr. Madhuri Sharon
Managing Director
Monad Nanotech Pvt. Limited
A702 Bhawani Towers,
Adishankaracharya Marg,
Powai Mumbai 400 076
Fax No +91 (0251) 2231029,
Tel No +91 (022) 25790633
Mobile No 9821238584
Email: monadnt@yahoo.com
Web: www. monandnano.com
Comp.Reg. No. U 24230 MH 2004 PTC 144302
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COTTON EXPERIMENTAL DATA |
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| CONCISED DATA OF SEEDLING GROWTH AND GERMINATION |
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|
Treatment |
Germination
% |
Length (cm ± S.E.) of |
IAA µg/ml |
Glutathione in Leaves |
|
Shoot |
Root |
|
CONTROL |
68% |
7.59
±0.014 |
6.80
± 0.06 |
6.36 µg/ml |
0.1766 |
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TREATED |
82% |
7.60
±0.020 |
5.78
±0.047 |
6.83 µg/ml |
0.2255 |
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IAA STAANDARD CURVE DATA |
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Standard IAA |
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µg / ml |
1 |
2 |
3 |
Mean |
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1 |
0.004 |
0.005 |
0.005 |
0.005 |
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2 |
0.008 |
0.014 |
0.011 |
0.011 |
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3 |
0.015 |
0.015 |
0.015 |
0.015 |
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4 |
0.03 |
0.029 |
0.031 |
0.03 |
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5 |
0.047 |
0.052 |
0.043 |
0.473 |
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6 |
0.067 |
0.083 |
0.065 |
0.716 |
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7 |
0.095 |
0.1 |
0.099 |
0.98 |
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8 |
0.13 |
0.123 |
0.125 |
0.126 |
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9 |
0.168 |
0.168 |
0.171 |
0.169 |
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10 |
0.192 |
0.2 |
0.197 |
0.196 |
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| GLUTATHIONE IN LEAVES OF COTTON |
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Cotton |
a |
b |
c |
d |
MEAN |
Remark |
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CONTROL |
0.1614 |
0.1618 |
0.1854 |
0.1979 |
0.1766 |
27.68% INCREASES |
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TREATED |
0.2004 |
0.2015 |
0.2018 |
0.2984 |
0.2255 |
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PROCEDURES FOLLOWED FOR |
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| IAA EXTRACTION & ANALYSIS |
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- IAA was analyzed using Tang and Bonner's method
- Freshly prepared Salkowski's reagent was used for detection
- 200 mg plant tissue was grinded with 5 ml of 80% chilled
methanol
- Extract was filtered through Whatmann paper no. 1
- Final volume of extract was made up to 10 ml using 80%
chilled methanol
- Optical density was measure after 30 minutes at 530 nm.
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| GLUTATHIONE EXTRACTION |
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| The glutathione extraction procedure was
as follows: |
- 5 gm of leaves was crushed in 5 ml of 80% chilled methanol
(as a solvent)
- Extract was sonicated for 10 min.
- After sonication the total volume was adjusted to 5 ml.
- Then 1ml of 5% tricholoroacetic acid was added to the
extract
- This sample was used for glutathione analysis.
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| GLUTATHIONE ANALYSIS |
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- The glutathione levels were estimated by Moron et.al's
(1979) method.
- Trichloroacetic acid was taken as blank.
- Procedure given in Biochimica et Biophysica acta, 582,
1979, 67-78 was followed.
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| INFERENCE |
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- Very significant increase in germination,
- Though seedling did not show any significant in crease
in shoot growth and even initial root growth was inhibited
in seedling BUT
- There was an increased level of auxin (IAA) in the
seedling of the blessed plants suggest an early establishment
of healthy plant.
- Absence of any type of disease (i.e. viral, fungal
and insect) was noticed in blessed plants, which is supported
by a 27.68% increase in the level of glutathione in them.
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Dr. Madhuri Sharon |
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GROWTH & GERMINATION OF SEEDLINGS |
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Sr. No. |
Length (cm) of 10 days old plant |
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Root |
Shoot |
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Blessed (B) |
Control (C) |
Blessed (B) |
Control (C) |
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1 |
6.0 |
6.4 |
9.3 |
9.6 |
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2 |
3.4 |
6.6 |
8.0 |
11.0 |
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3 |
9.5 |
11.0 |
11.2 |
9.6 |
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4 |
10.4 |
10.7 |
9.2 |
6.6 |
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5 |
8.7 |
4.9 |
9.4 |
9.0 |
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6 |
9.2 |
7.2 |
10.0 |
7.8 |
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7 |
8.1 |
7.0 |
8.3 |
8.0 |
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8 |
1.2 |
7.0 |
10.0 |
11.0 |
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9 |
10.8 |
10.0 |
8.3 |
6.5 |
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10 |
4.0 |
11.0 |
9.1 |
12.0 |
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11 |
4.5 |
5.0 |
8.2 |
8.5 |
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12 |
5.7 |
7.5 |
9.6 |
8.0 |
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13 |
5.5 |
7.0 |
5.6 |
11.0 |
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14 |
7.7 |
8.0 |
9.0 |
11.0 |
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15 |
5.3 |
7.7 |
8.4 |
10.4 |
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16 |
4.0 |
6.0 |
6.3 |
5.6 |
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17 |
9.5 |
7.0 |
8.1 |
6.2 |
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18 |
7.4 |
11.5 |
8.0 |
4.7 |
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19 |
7.9 |
5.3 |
8.2 |
8.2 |
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20 |
7.2 |
4.1 |
7.0 |
11.2 |
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21 |
6.8 |
2.2 |
5.1 |
9.0 |
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22 |
8.1 |
10.9 |
5.5 |
10.2 |
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23 |
7.0 |
6.0 |
8.9 |
11.0 |
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24 |
4.1 |
6.2 |
9.4 |
2.0 |
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25 |
4.5 |
5.0 |
9.1 |
4.6 |
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26 |
5.2 |
6.8 |
7.0 |
4.3 |
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27 |
7.0 |
9.1 |
9.3 |
6.2 |
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28 |
3.9 |
6.4 |
5.6 |
10.2 |
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29 |
11.1 |
12.0 |
7.2 |
7.6 |
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30 |
4.1 |
3.3 |
7.2 |
1.5 |
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31 |
5.3 |
4.3 |
10.0 |
7.4 |
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32 |
5.3 |
6.0 |
6.7 |
1.6 |
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33 |
4.0 |
2.3 |
6.4 |
3.2 |
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34 |
4.0 |
3.0 |
6.0 |
3.5 |
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35 |
5.5 |
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7.9 |
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36 |
4.5 |
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6.5 |
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37 |
4.4 |
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7.0 |
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38 |
3.5 |
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2.1 |
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39 |
4.5 |
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9.5 |
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40 |
3.5 |
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2.5 |
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41 |
2.8 |
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2.0 |
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42 |
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43 |
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44 |
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45 |
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46 |
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47 |
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48 |
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49 |
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50 |
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Mean |
5.78 |
6.80 |
7.60 |
7.59 |
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